HSP90 Kit ELISA

N° du produit ABIN2043617

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain HSP90.

Aperçu rapide pour HSP90 Kit ELISA (ABIN2043617)

Antigène: HSP90 (Heat Shock Protein 90 (HSP90))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12-200 ng/mL

Application: ELISA

Détail du produit HSP90 Kit ELISA

Seuil minimal de détection: 3.12 ng/mL

Marque: BioAssay™

Sensibilité: < 1.28 ng/mL

Ingrédients: A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL

Information d'application

Plaque: Pre-coated

Protocole: The Human Heat Shock Protein 90 (HSP90) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HSP90 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP90. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HSP90. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSP90, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HSP90 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: HSP90 (Heat Shock Protein 90 (HSP90))

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals